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Was there anything in the experimental procedure that may have biased or limited the results? What changes to the methods or analysis could have improved the results and what additional experiments would you have done? Was there any information not provided in the manuscript that you feel would have improved the study?

This study did not examine the gut microbiome, which is known to be critical for human health. Based upon the results of the study, what do you think would be the interaction between the gut microbiome and ISS?

The study shows that the saliva microbiota was the most affected body site of those sampled. What are some possible reasons for this finding?

The study admits that examining only what microbes are present does not provide a complete picture. How would you examine what biological processes are occurring and what could cause these processes to change? Is this something that could be continuously monitored while in long term space travel?

What do the findings of the study mean for long term travel in space and what steps would you take to promote maintenance of human health before, during and after flight?

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