Biology 107 Assignment 3 Part B: Writing a Results and Discussion for Enzymes Data

This is individual work. Improper collaboration is a violation of the Academic Integrity Policy. Do NOT share any of your work with other students in the course as this inevitably leads to improper collaboration. You need to share your data, but you should not be sharing your excel sheets that contain your work, or any other documents.

·The page limit is 4 pages. Instructors will stop reading text at the end of the 4th page. This page limit does not include your full reference section or graphs.

·Your instructor will not grade this assignment if you have not uploaded the Academic Integrity Training Certificate

Make sure you read the document about how to write a Discussion (located in the assignment folder); it provides examples of excellent, average, and needs significant improvement lab reports. Reading these will help you model how to write a good Discussion.

Formatting:

·The assignment must be typed, 12pt font — Arial, Calibri, or Times New Roman, double spaced with 1” (inch) margins.

·Do not include a separate title page

·Include heading titles (ex: Results, Discussion, References, Figures,Appendix)

·Formatting errors will result in deductions
·Your assignment must be uploaded as a PDF. Failure to do this will result in a mark deduction.

To avoid plagiarism, make sure you review the materials from the Academic Integrity folder and remember: Cite! Cite! Cite! Your discussion should be filled with citations! Also remember: Paraphrase! Paraphrase! Paraphrase! Write everything in your own words! Simply rearranging words, and swapping out a few words is not paraphrasing. Failure to cite or paraphrase will result in an Academic Integrity Violation Report. In science writing although using quotations (with quotation marks and a citation) is not an academic integrity issue, it is not done in our discipline. We expect you to synthesize the information and therefore have the ability to use your own words, and then cite the source of that information.

Time management and meeting deadlines are important skills to learn while at University. Don’t wait until the night before to start your lab report!

Tips:
· Writing is challenging for many students, it will get easier the more you do it (we promise!).

· Written communication is an essential skill for all careers, all students benefit from having more writing assignments (trust us!). Although this is format is specific to Biology writing, regardless of which career path you choose, learning a format and communicating effectively in that format is a durable skill.

·If you do not understand the purpose of the experiment, how enzymes work, how pH affects protein folding, etc, it will be very hard to write this lab report. Putting in the time and effort to understand before you even start writing will have a large pay off.
·Don’t start your lab report the night before it is due. Lab reports take time and it’s a great idea to leave time for many revisions
·Organize ideas into paragraphs! Your Discussion should not be one mega-paragraph. A good paragraph should start with a topic sentence and only cover one idea. The

sentences in the paragraph should provide explanation of the one idea, examples, and/or evidence to back up the topic sentence.
·
There are papers posted on Blackboard. Use these to strengthen your discussion. Only use the parts of the paper that you understand. Including information that you do not understand will not make your paper better (in fact, it almost always has the opposite effect!).

Your lab report will contain the following (and be sure to include the headings: Results, Discussion, References, Graphs):

Results

·Written results for both of the experiments you did in Lab 3. One paragraph for the results from Part A (use the class data found in your instructor’s folder), and another paragraph for Part B (the data you collected with your group-make sure you do not forget to share with each other in a timely fashion).

·You practiced writing a Results section in Assignment 2. Apply your instructor’s feedback, and refer back to the Assignment 2 materials if you forget how to write a good Results section.
·Don’t forget to compare your results to the control and refer to your figures.

·For the purpose of practice, we will assume our replicates for Part A are biological. Incorporate the statistical results from t-tests to support your results for Part A. In terms of which means you compare, think about what you want to say in your results. For example, If you want to say the pH1 is significantly different from the control, support this with a p-value from a t-test. If you think the rates between pH7 and pH8 are not significantly different, support this with a p-value.

·** you do not need to do t-tests for your data from Part B

·Do not paste the graphs in this section, figures are attached at the end of the references section of your report.

Discussion

1. Conclusions and in-depth biological explanation for the first experiment.

o One paragraph should state the conclusions from Part A. Conclusions are an interpretation of results. Recall from the Scientific American article with the stats assignment that p-values are not meant to be the gatekeeper, p-values are not the final verdict, but a tool. If experimental design concerns (ex: large standard deviation in the replicates) mean the validity of the p-values or confidence intervals are questionable, it is good to indicate that when you draw conclusions. Ex: Although the difference was not statistically significant, this could be due to …….

oDo these findings support or refute your hypothesis?

oYour next paragraph should provide a comprehensive and in-depth explanation of the results with respect to your knowledge of enzymes and pH. Use your knowledge of enzymes and protein folding to explain how pH affects the rate of reaction.

oUse the literature we provided in the assignment folder to support this

·It’s important to find evidence from another researcher to support your findings (the papers provided can help you do this) but be specific in how what they found supports or refutes the conclusions from your data. (ex: Smith et al, 2015 also found that…..)

2.Conclusions and in-depth biological mechanism for the second experiment (the

experiment you designed).

3. Reflection on the variation

Large variation in the data can limit the conclusions we draw. Reflect on the variation in your data and identify plausible sources of variation for both Part A and Part B. Be sure to explain how this would affect variation and connect this to the error bars.

References

·Make sure the reference list is alphabetical and follows the format outlined in the lab report guidelines (found in the assignment folder)

·Make sure in text citations are provided in places they are necessary and follow the correct format. When in doubt, cite.

Graphs

1.Graph for the rate of reaction for Part A (effect of pH). Your instructor will post the class data with all of the rates, you will need to determine the mean and make confidence interval error bars.

2.Graph that effectively communicates the results of the experiment you designed (Part B- be sure to report your data as rates in nMol/min, not the raw data (which was concentration vs time). Be sure to use the means for your replicates and include standard deviation error bars.

**Please note that you are not including a figure legend for these graphs to keep this assignment more streamlined. You’ve had two assignments to practice writing figure legends before your final lab report assignment.

Appendix

Upload the excel file that contains your work (do not make this into a PDF, we need to be able to open your actual excel file to check your work). Your instructor should be able to open the excel file and clearly see:

·

oYour work in calculating means and CI for Part A

oYour work in determining p-values for Part A (label things clearly so your instructor does not need to guess at what you did!)

oYour work in calculating means and standard deviation for Part B

The post Biology 107 Assignment 3 Part B: Writing a Results and Discussion for Enzymes Data This is individual work. Improper collaboration is a violation of the Academic Integrity Policy. Do NOT share any of your work with other students in the course as this inevitably leads to improper collaboration. You need to share your data, but you should not be sharing your excel sheets that contain your work, or any other documents. appeared first on My Academic Papers.